Capto HiRes ion exchange chromatography columns

Capto HiRes Q and Capto HiRes S are prepacked columns for high-resolution purification of proteins and other biomolecules by ion exchange chromatography. They are well-suited for the preparation of highly pure proteins for structural studies and for protein analysis.

• High resolution columns—for laboratory scale ion exchange chromatography.
• Suitable for preparing highly pure proteins—essential in, for example, structural studies using techniques such as cryo-electron microscopy or protein crystallography.
• Suitable for protein analysis in research or quality control—for example, for characterization of target protein charge variants.
• High quality column—excellent reproducibility, durability, and high chemical and pH stabilities.
• Replacement for Mono Q and Mono S columns—delivering similar or improved resolution, with easy transition from one column platform to the other.

Capto HiRes ion exchange chromatography resin
The chromatography resins packed in Capto HiRes Q and Capto HiRes S ion exchange columns are based on a high-flow agarose base matrix and a small bead size (average bead size 9 μm).

Capto HiRes Q and Capto HiRes S are strong anion and strong cation exchangers, respectively with ion exchange properties that remain constant during chromatography within the recommended pH range of 2 to 12.

A high-performance IEX column format
Capto HiRes Q and Capto HiRes S IEX resins are prepacked in high-performance Tricorn glass columns of two different sizes. The columns have fittings for simple connection to ÄKTA chromatography systems or equivalent high-performance systems. All column parts are biocompatible.

High-resolution protein purification
In many research areas, for example in structural biology using X-ray crystallography or cryo-electron microscopy (cryo-EM), obtaining homogeneous size and charge of biomolecules is crucial for the elucidation of their structures. High-resolution separation of samples based on their charge properties is essential to secure sample charge homogeneity and success of the study. Capto HiRes columns separate proteins and biomolecules that are very close in net charge, which makes these columns great for these applications.

Protein analysis
Capto HiRes ion exchange columns can be used for protein analysis, in applications such as characterization of target protein charge variants.

Capto Q HiRes and Capto S HiRes replace MonoBeads columns
A separation that worked on a Mono Q or Mono S column may be performed on a Capto HiRes Q or Capto HiRes S column with little modification or optimization. Similar resin selectivity and slightly improved resolution can be expected with the Capto HiRes columns while using the same experimental conditions. The similar selectivity of the two columns ensures a smooth transition even for quality control (QC) applications.