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Cardiolipin IgG/IgM
High Quality Assays with Reproducible and Reliable Results
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Anti-Cardiolipin IgG / IgM ELISA is a test system for the quantitative measurement of IgG and IgM class autoantibodies against cardiolipin in human serum or plasma.
This product is intended for professional in vitro diagnostic use only.
Highly purified cardiolipin is coated on microwells saturated with beta-2-glycoprotein I. The determination is based on an indirect enzyme linked immune reaction with the following steps:
- Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells.
- After incubation, a washing step removes unbound and unspecifically bound serum or plasma components.
- Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes.
- After incubation, a second washing step removes unbound enzyme conjugate.
- After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue colored product.
- Addition of an acid stops the reaction generating a yellow end-product. The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm.
