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Cas9 Nuclease Protein
Cas9 Nuclease protein NLS, in complex with guide RNA, offers benefits for CRISPR/Cas9 technology over plasmid or mRNA transfection: Off-target mutations rarely occur Prolonged expression of Cas9 increases off-target editing but RNP delivery is naturally transient Transfection/injection is easy Cas9-NLS bypasses transcription and translation by delivering the protein directly The efficiency of the guide RNA in vit…
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Cas9 Nuclease protein NLS, in complex with guide RNA, offers benefits for CRISPR/Cas9 technology over plasmid or mRNA transfection:
- Off-target mutations rarely occur
- Prolonged expression of Cas9 increases off-target editing but RNP delivery is naturally transient
- Transfection/injection is easy
- Cas9-NLS bypasses transcription and translation by delivering the protein directly
- The efficiency of the guide RNA in vitro is measurable
- The RNP complex can be tested with a cleavage assay in vitro before it is used in cell culture or in vivo
- Assay is DNA-free
- No risk of DNA integration that can happen using plasmids plus no cloning required
