Cell Counting Kit, 30 dual-chambered slides, 60 counts, with trypan blue

Use these dual-chamber slides for fast, highly reproducible cell counts using the TC10™ or TC20™ Automated Cell Counters. The kit contains filter-sterilized 0.4% (w/v) trypan blue in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution.

• Counting parameters – range of 5 x 104 – 1 × 107 cells/ml and 6 – 50 μm cell diameter
• Each chamber only requires 10 μl of suspended cells
• Volume counted in each chamber is 0.4 μl (equivalent to four 1 x 1 mm squares of a manual hemocytometer)
• Both chambers can be loaded prior to insertion
• Chambers labeled A and B, with arrow next to chamber indicating direction of slide insertion
• Can be used with or without trypan blue

The counting slides are disposable plastic slides with dual chambers composed of polymethyl methacrylate (PMMA). Each slide is 75 × 25 × 1.8 mm (W x D x H) with a chamber depth of 100 μm.