Cell Death Detection ELISA

Roche Cell Death Detection ELISA - Photometric enzyme immunoassay for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death

Benefits of Roche Cell Death ELISA:

• High sensitivity: (5 x 10² cells/ml)
• Fast performance: (5 - 6 hours)
• No prelabeling of cells necessary
• Nonradioactive assay system
• No species restrictions
• Easy handling
• Low background
• Function-tested

Product description - Roche Cell Death Detection ELISA

Cell Death Detection Assay time: 5 - 6 hours
Negative control: Depending on cell-culture conditions, each exponentially growing permanent cell culture contains a certain amount of dead cells (typically approximately 3 - 8%). In the immunoassay, these inherent dead cells in the untreated sample (without a cell-death-inducing agent) will cause a certain absorbance value (negative control).
Sensitivity: The exact detection limit of dying/dead cells in a particular sample strongly depends on the kinetics of cell death, the cytotoxic agent used, and the amount of affected cells in the total cell population. Using HL60/camptothecin (CAM) as a cellular model system for cell death, the immunoassay allows the specific detection of mono- and oligonucleosomes in the cytoplasmic fraction of 5 x 10² cells/ml (= 50 cell equivalents/well).
Specificity: Anti-histone antibody reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, Xenopus). Anti-DNA-peroxidase (POD) antibody binds to single- and double-stranded DNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems.
Sample material: Cytoplasmic fractions (lysates) of cell lines, cells ex vivo, or tissue homogenates.