Cell Organelle Toolbox

Being able to visualize endogenous cellular structures in live cells is key for many high content analyses. The two Chromobodies® of the ChromoTek’s Cell Organelle Toolbox offer a new way to investigate key compartments of the cell non-invasively.

Lamin-Chromobody®
The new Lamin-Chromobody® is a major breakthrough especially in High-Content Analysis (HCA) as apoptosis assays thus far rely on static endpoint assays.

The nuclear lamina is a meshwork enclosing the entire nucleus - the control center of eukaryotic cells. Its function is to hold together nuclear content and to add mechanical support to the nucleus. The nuclear lamina is a very sensitive subcellular structure. It has to maintain certain rigidity and at the same time allow structural dynamics. Therefore, artificial incorporation of overexpressed and fluorescently labeled lamin protein mostly leads to undesired cytotoxic side effects. The new Lamin-Chromobody® overcomes these unwanted side effects:

• Visualize the nuclear lamina without interfering with its cellular functions.
• Monitor the nuclear integrity and morphology during live cell microscopy as an indicator for cellular health and viability.
• Use the Lamin-Chromobody® as biosensor for real-time assays of mitosis or apoptosis.

Actin-Chromobody®
Your new tool for detection of actin in live cells, the Actin-Chromobody®, allows you to monitor the cytoskeleton in real time. Taking advantage of their small size the Chromobody® works even in the cytoplasmatic environment inside of living cells.
• Easily transfect into cells and non-invasively visualize actin cytoskeleton in real time
• Target and trace microfilaments in your cell line of interest
• Use Actin-Chromobody® as a marker of cellular morphology and as a probe for cellular motility
• Actin-Chromobody® does not affect cell viability or migration.