Products & ReviewSpectroscopy

Chirascan™ Q100 Circular Dichroism Spectrometer

Applied Photophysics LtdAvailable: Worldwide

Unmatched Productivity and Performance 

Applied Photophysics Ltd

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The Chirascan Q100 Circular Dichroism (CD) Spectrometer offers comprehensive insight into the Higher Order Structure (HOS) characteristics of complex biomolecules. High-performance CD spectrometry and reproducible robotics combine to produce quality data compatible with the most strict statistical analysis techniques. The outcome: objective, statistically-validated HOS comparisons. Fully integrated for unmanned operation, the Chirascan Q100 saves days of operator time, analyzing 48 buffer-sample pairs in 24 hours whereas a skilled operator can process only up to 14 samples per day on a manual system.

Ready to run

The Chirascan systems are provided with the features and accessories needed for acquisition of superior quality data from ‘day one’.

Molecular Sieve, Activated Charcoal Filter

  • Removes common gas impurities

Active Nitrogen Management System

  • Software-controlled
  • Regulates purge gas consumption

Air-Cooled Xenon Lamp

  • Up-time recorded
  • Software-controlled

Monochromator

  • Dual polarizing prisms maximize light throughput
  • Generates horizontally, linearly polarized monochromatic light

Photoelastic Modulator

  • Converts horizontally polarized light to circularly polarized light. Alternates between left- and right-handed circular polarized light

Integrated Autosampler

  • Precise liquid handling and reproducibility
  • Eliminate sample handling errors
  • Temperature-controlled storage maintains sample integrity

Temperature-Controlled Sample Chamber

  • Continuous temperature ramps (single sample mode)
  • Consistent analytical conditions

Water Circulator

  • Dissipates heat from sample chamber and sample storage Peltiers

Avalanche Photodiode Detector

  • Maximum sensitivity (high signal: noise)

Optics-Based, Multiwave­Length Calibration

  • For CD accuracy at every wavelength

Fixed Flow Cells

  • Eliminates errors of cuvette handling
  • Choice of pathlength to optimize concentration and absorbance
  • Recognized by Chirascan Control to choose run/wash/dry protocols

Cuvettes and Holders

  • Selected for far- and near-UV CD analysis of biomolecules (single sample mode)

Control and Analysis Software

  • Saves time with scheduled start-up/shutdown of lamp and N2 supply
  • Easily defined run parameters and store standard protocols
  • Ensures O2-free conditions with N2 purge
  • Fail-safe lamp switch-off if N2 flow drops
  • Recognizes flow cell to choose ideal run/wash/dry protocol

HOS Comparison Software

  • Create statistically-validated ­comparisons

Global Thermodynamic Analysis

  • Obtain melting points and enthalpies from multi-wavelength, thermal denaturation experiments (single sample mode)

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