CleanDTR
BigDye terminator removal sequencing clean up kit
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Cleaning DNA samples
These beads are easy to use, and are extremely effective in our applications. The beads produce high quality results and also produce extremely reproducible work. My research would not be the same without these cost-effective and efficient beads. I also appreciate how friendly and the willingness to help that all of the sales team has at GC Biotech to help provide me with what I want.
Review Date: 16 Jan 2020 | CleanNA
CleanDTR is an efficient paramagnetic bead-based system, designed to remove unincorporated dye terminators from Sanger sequencing reactions. The CleanDTR process involves three simple steps including bind, wash and elute. While binding the sequencing product selectively to the magnetic particles, unincorporated dyes, nucleotides, salts and primers will be removed during ethanol washes. This principle allows for elution of the pure Sanger Sequencing product in the elution buffer of choice. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Features:
- Long Phred 20 read lengths averaging over 800 bps
- Pass rates over 85% or higher
- Efficient elimination of sequencing reaction contaminants
- Reduce BigDye* usage, due to increased average signal strenght
Available models: CDTR-0005, CDTR-0050, CDTR-0500