CleanSEQ - Dye Terminator Removal
The Agencourt CleanSEQ system is a rapid, high performance dye-terminator removal process based on Solid Phase Reversible Immobilization (SPRI) technology.
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The Agencourt CleanSEQ system is a rapid, high performance dye-terminator removal process based on Solid Phase Reversible Immobilization (SPRI) technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performed manually or fully automated for high throughput dye-terminator removal. The Agencourt CleanSEQ system produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology. The Agencourt CleanSEQ protocol does not require precipitation, filtration or centrifugation. The Agencourt SPRI technology is easily scaled and automation friendly, allowing both high throughput and format flexibility. Agencourt Bioscience has written and optimized software methods for Beckman Coulter's Biomek Laboratory Automation Workstations.
Features:
- Long Phred 20 read lengths averaging over 700 bps
- Pass rates of 85% or higher Increased average signal strength
- Efficient elimination of sequencing reaction contaminants
