cOmplete Lysis-B (2x), EDTA-free

Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC.

Application

Use Complete Lysis-B (2x), EDTA-free to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteases, including serine proteases and cysteine proteases, but not metalloproteases.

The EDTA-free version of Complete Protease Inhibitor Cocktail Tablets in the kit is used to inhibit proteolytic activity in cases in which EDTA may interfere with protein stability or subsequent assays or purification methods (e.g., Immobilized Metal Chelate Affinity Chromatography [IMAC]).
The Lysis-B Reagent purifies inclusion bodies to near-homogeneous levels, but does not solubilize them. The extracted proteins are suitable for further purification or analysis in downstream applications.

Benefits

• Save time with a method that achieves fast, complete, and gentle lysis of bacteria in only 10 minutes.
• Obtain high yields of extracted proteins.
• Ensure protein integrity by using reagents that do not denature or interact with proteins.
• Extract soluble proteins and purify inclusion bodies.
• Extract recombinant proteins from insect cells infected by baculovirus.
• Achieve non-toxic, effective protection of proteins against a multitude of proteases (serine proteases and cysteine proteases, but not metalloproteases).
• Employ reagents that are suitable for Immobilized Metal Chelate Affinity Chromatography (IMAC) and downstream assays.

Product Description

The Complete Lysis-B (2x), EDTA-free bacterial protein extraction reagent contains a mild, double-concentrated non-ionic detergent that enables very efficient and gentle extraction of proteins, especially recombinant proteins, from bacteria (e.g., E. coli) in small volumes. This simple extraction method completely eliminates the need for mechanical disruption (e.g., standard sonication). Rapid cell lysis occurs in just 10 minutes at room temperature. The protein yields obtained with this kit are significantly higher compared to those obtained by using sonication.

In combination with Complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets, included in the kit, the Lysis-B Reagent provides a very convenient and efficient way to extract proteins and simultaneously inhibit protease activity during the initial extraction steps. Complete Tablets eliminate the time-consuming search for the right protease inhibitor. The ready-to-use water-soluble, non-toxic tablets work optimally with the kit’s Lysis-B Reagent. The extracted proteins can be further purified or analyzed in downstream applications.

Contents

100 ml Lysis-B Reagent for protein extraction from bacterial cells (2x concentrated)
20 Complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets supplied in EASYpacks (each tablet is sufficient for 5 ml of extract)