cOmplete, Mini, EDTA-Free

Protease Inhibitor Cocktail Tablets supplied in EASYpacks

Application

Used for the inhibition of proteolytic activity in small volumes in which EDTA may interfere with protein stability or subsequent assays or purification methods (e.g., Immobilized Metal Chelate Affinity Chromatography [IMAC]).

Benefits

• Simplify protection.
  Proteins are protected against a multitude of proteases by simply adding a Complete Tablet to aqueous protein isolation buffer.
• Use a convenient tablet.
  Tablets combining different protease inhibitors, with or without EDTA, are available for either 10 ml or 50 ml of lysate.
• Eliminate weighing small amounts.
  Sufficient amounts of various inhibitors are conveniently supplied in a tablet form.
• Insist on a versatile product.
  Proteases are inhibited in extracts from almost any tissue or cell type, including animals, plants, yeast, bacteria, or fungi.

Product Description

Starting concentration: 1 tablet contains protease inhibitors sufficient for a 10-ml cell extract.
Specificity: Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
Solubility/Stability: Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at +2 to +8°C, or at least 12 weeks at -15 to -25°C. Can be used in thiol-containing solutions at room temperature.
Note: Inhibition efficiency is quickly and sensitively tested with the Universal Protease Substrate (Cat. Nos. 11 080 733 001 or 11 734 334 001).