Corning® ITS Premix Universal Culture Supplement, 20 mL, 1/Pack
Corning® ITS Universal Culture Supplement, 20 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 20 mL (20L equivalent), 1/packThe ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin (5u…
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Cell culture
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Review Date: 16 Feb 2021 | Corning Life Sciences
Corning® ITS Universal Culture Supplement, 20 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 20 mL (20L equivalent), 1/pack
The ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin (5ug/ mL), Transferrin (5ug/ mL), and Selenious Acid (5ng/ mL)
Formulation
Corning ITS Premix -Lyophilized from a water solution; reconstitute in 20 mL (cat. no. 354350) or 5 mL (cat. no. 354351) dH2O (stock solution)
Quality
- Tested for ability to promote proliferation of HeLa cells in serum-free medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Preparation and Storage
Corning ITS Premix - Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Avoid multiple freeze/thaws.
Tip
Corning ITS Premix - Recommended dilution: 1:1000 (dilute stock solution in serum-free medium)