CORTISOL EIA KIT
This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of cortisol in biological samples including urine, saliva, and plasma. Briefly, cortisol in the samples or standards competes with cortisol conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for cortisol coated on the microplate. The HRP activity results in brilliant blue color development when the substrate…
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Great product
Analyze cortisol concentration in blood samples
It is easy to use by following the protocol, and the result is reproducible.
Review Date: 4 Feb 2022 | Oxford Biomedical research
This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of cortisol in biological samples including urine, saliva, and plasma. Briefly, cortisol in the samples or standards competes with cortisol conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for cortisol coated on the microplate. The HRP activity results in brilliant blue color development when the substrate is added, with the intensity of the color development proportional to the amount of cortisol-HRP bound and inversely proportional to the amount of unconjugated cortisol in the samples or standards.
When measuring urine samples, it is recommended to normalize samples to creatinine to account for variations in fluid intake and excretion. Creatinine is excreted at a constant rate and can be used to normalize the rate of excretion of other analytes.
