COVID-19 Human IgM ELISA Kit
RayBio® COVID-19 S-Protein S1 Receptor Binding Domain Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Place your order directly with the manufacturer.
Antigen InformationGene Symbols:
IGM
Assay FormatNumber of Targets Detected:
1
Species Detected:
Human
Compatible Sample Types:
Serum
Design Principle:
Indirect
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative; Semi-Quantitative
Solid Support:
96-well Microplate
Product SpecificationsSize:
1, 2, or 5 x 96-Well Strip Microplate Kit
Application NotesKit Components
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Negative Control
- HRP-anti positive control IgM Antibody
- HRP-anti Human IgM Antibody
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 μl positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
Add 100 μl prepared HRP-anti positive control IgM Antibody and/or 100 μl of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
