COVID-19 Spike Protein ELISA
SARS-CoV-2 (COVID-19) Spike Protein ELISA Kit. Detects the S-Protein. Standard protein code: 230-01103.
Place your order directly with the manufacturer.
Antigen InformationGene Symbols:
S
Protein Name & Synonyms:
SARS-CoV-2 Spike Protein, S2 Subunit, S protein, spike protein
Assay FormatDetection Range:
2.7 ng-ml - 2000 ng/ml
Recommended Dilution (serum & plasma):
0
Sensitivity:
2.7 ng/ml
Specificity:
This ELISA antibody pair detects virus COVID-19 S Protein (S2 subunit).
Number of Targets Detected:
1
Species Detected:
Virus
Compatible Sample Types:
Cell Culture Supernatants; Serum
Design Principle:
Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate
Product SpecificationsSize:
1, 2, or 5 x 96-Well Strip Microplate Kit
Standard CurvesSpiking & Recovery ResultsLinearity ResultsApplication NotesKit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
