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Custom Trimer Phosphoramidite Mixes

Glen ResearchAvailable: Worldwide

Tools for fine-tuning protein function

Glen Research

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Glen Research offers a unique line of products known as trimer phosphoramidites. These are trinucleotide reagents that cover all 20 amino acids and allow mutation of a gene at the codon level rather than at individual bases. If you’re still using degenerate oligonucleotides for mutagenesis library construction, it might be a good time to consider using trimer phosphoramidites.

  • For researchers looking to generate oligonucleotide libraries for mutagenesis, these reagents avoid stop codons and amino acid redundancy
  • The trimer phosphoramidite approach to mutagenesis library construction is distinguished from other methods by the ability to fully control total or partial randomization at each codon position of a gene, maximizing the efficiency of high throughput screening processes
  • Trimer phosphoramidites can be added during synthesis using standard DNA synthesis chemistry

All of the trimers are available individually so that researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are available: 13-1991-xx, for incorporating all 20 amino acid codons equally into a sequence and 13-1992-xx, for incorporating 19 amino acid codons (-Cys).

For a custom trimer mix of a particular subset of codons or a trimer mix that represents a set of trimers that is biased toward a particular codon or codons, please contact support@glenresearch.com for a quotation and projected delivery date.

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