Cytek® Amnis® CellStream® Flow Cytometer
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Avoid avoid avoid
Cell phenotyping
Massive waste of money - this machine does not produce usable data. It is incompatible with FlowJo - you are locked into a terrible software solution. The overlap between channels is extremely difficult to resolve. Compensatation of Sec channels (you read that correctly) means that you cannot run cells of different Fsc and SSca properties. Shocking post-sale support from Bio-Rad (original manufacturers). Years of back and forth with no working solution, until I gave up. The only saving grace is that technically my interactions were with Bio-Rad and not with the new owner Cytek. Out of 30+ users in our department, there are vague reports of stains having worked, but I investigated the data is destroyed by artefacts - unpublishible data. Massive learning curve, with little or no support. The engineers know this is a bad product that should never have seen the light of day. Can't even use it to count cells as it is impossible to resolve cells from 'other' events that could be debris who knows? This machine lies unused and abandoned for a very good reason. There are 20-year-old cytometers being used in the same room. If anyone starting a tenure process is even considering this instrument, please contact me. I would go as far as refusing this instrument if no other option were made available. Design your tenure process so that you avoid this instrument. A pair of dice would tell you more about your cells.
Review Date: 11 Apr 2024 | Cytek Biosciences
Cytek® Amnis® CellStream® benchtop flow cytometry systems are highly-customizable, compact flow cytometers that use our patented time delay integration charge-coupled device (TDI CCD) camera technology for detection, offering exceptional sensitivity for studying small particles. Combined with intuitive software and high-throughput acquisition, Amnis CellStream systems allow you to resolve complex cell populations and view cells as they are analyzed in real time—making quality control and obtaining consistent results easier than ever.
The fully configurable CellStream system enables you to obtain reproducible, multi-parametric, single-cell data for a wide variety of applications.
