Cytomegalovirus (CMV) IgG ELISA
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An enzyme immunoassay for the detection of IgG antibodies to cytomegalovirus (CMV) in serum. Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and ummunosuppressive therapy. About half the pregnant women who contract a primary infection spread the disease to their fetus. When acquired in-utro, the infection may cause mental retardation, blindness, and/or deafness. Serological test for detecting the presence of antibody to CMV can diagnose active or recent infection and provide valuable information regarding the history of previous infection. These tests are also useful in screening blood for transfusions in newborns and immunocompromised recipients. CVM IgG ELISA is an accurate serologic method to detect CVM IgG antibody for identification of CVM infection.Purified CMV antigen is coated on the surface of microwells. Diluted sample specimen serum is added to the wells, and the CMV IgG specific-antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of CMV IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrators and controls.
