Cytomegalovirus (CMV) IgM ELISA
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An enzyme immunoassay for the detection of IgM antibodies to cytomegalovirus (CMV) in serum. Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions andimmunosuppressive therapy. About half the pregnant women who contract a primary infection spread the disease to their fetus. When acquired in-utero, the infection may cause mental retardation, blindness, and/or deafness. Serological tests for detecting the presence of antibody to CMV can diagnose active or recent infection and provide valuable information regarding the history of previous infection. These tests are also useful in screening blood for transfusions in newborns and immunocompromised recipients. The CMV IgM ELISA is an accurate serologic method to detect CMV IgM antibody for identification of CMV infection.Purified CMV antigen is coated on the surface of microwells. Diluted specimen sample serum is added to the wells, and the CMV IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solutionof TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific- antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.Purified CMV atigen is coated on the surface of microwells. Diluted specimen sample serum is added to the wells, and the CMV IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated in the sample is proportional to the amount of IgM specific-antibody in the sample. The results are by a microwell reader compared in a parallel manner with calibrator and controls.
