Dispase® II (neutral protease, grade II)

Application

Dispase is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid and effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.

Benefits

• Rapid, effective, yet gentle agent that liberates cells with minimal cell damage.
• Maintain cell membrane integrity.
• Non-mammalian source - free of mycoplasma and animal virus contamination.
• Extremely stable to influences of temperature, pH, and interference by serum components.
• Easily inactivated by chelating agents or by dilution.
• Delivers higher activity and convenience.