DNase I
DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyses phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'- phosphate and 3'- OH groups.
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Great product for the price, ease of delivery, and good customer service as always!!
DNA isolation & WGS Preparation
This product arrived overnight and works wonders at digesting RNA in my DNA samples that I prepared for WGS!
Review Date: 25 Mar 2022 | VWR International Ltd
The enzyme activity is strictly dependent on Ca²+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg²+, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion. In the presence of Mn²+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt ends or with one or two nucleotide overhangs.
