Dual-Glo® Luciferase Assay System
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…
Protein expression
We have been using the Dual-Glo assay system for years. It is highly sensitive and reproducible. The downside is the price tag.
Review Date: 16 Nov 2021 | Promega Corp.
Analyze mammalian cell samples
Very easy to use, clear protocol, reproducible results in a convenient plate format.
Review Date: 31 Oct 2019 | Promega Corp.
Analyze activation of reporter system in cell line
It was very easy to use to get the answer I wanted.
Review Date: 25 Oct 2019 | Promega Corp.
This kit is easy to use with great depletion of firefly signal.
Review Date: 28 Jan 2011 | Promega Corp.
Very expensive!
Review Date: 18 May 2009 | Promega Corp.
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample.
This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false positive and false negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.