Miniaturized GloSensor cAMP Assay by Promega, Using the Echo® Liquid Handler

As biological assays used for HTS and uHTS are miniaturized, it is critical to maintain assay sensitivity while minimizing reagent volumes and maximizing throughput. The GloSensor cAMP Assay provides an extremely sensitive and easy to use, real-time luminescent assay format for the interrogation of over-expressed or endogenous GPCRs that signal via changes in the intracellular concentration of cAMP. This scientific poster demonstrates optimization of the GloSensor cAMP Assay in low-volume format using a stably transfected HEK293 cell line model expressing the melanocortin 4 receptor (MC4R) and a cAMP-sensing variant of firefly luciferase.