ECM 830 Square Wave Electroporation System
Versatile electroporation system suitable for applications for gene, drug and protein delivery including: mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant, nuclear transfer and CRISPR NEW! Now available with Pulse Switcher for automatic polarity switching!
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Sturdy & versatile electroporator for mammalian & bacterial electroporation.
Electroporation of cells
An easy to use Electroporation device for mammalians cells and bacteria. The system consists of the ECM 830, a square wave pulse generator which can be connected to a safety stand based cuvette holder / variety of other electrodes like petri pulser electrodes (for 35mm dishes). There is also a footstand switch for foot based electroporation control. The electroporation voltage, duration, pulse etc can be controlled by the simple menu and the knob to select the options. (Newer version have a touch screen interface). A wide variety of prevaricated protocols are available for different tissue and cell types, Mostly we have cuvette based electroporation with the BTX supplied electroporation cuvette. The cuvettes come in pre-sterilised packs along with a small disposable plastic pipette for transfer of cells into/out of the cuvette. The BTXpress electroporation provides a ready to use buffer with less arcing for most cell types. Other electroporation buffers can also be used, but test these prior to actual use for electrical arcing. Overall the electroporator is well built, and easy to use. It has been working fine for us without any issues for last 6 plus years. Mostly we have been using this to successfully deliver plasmid DNA. The support from BTX Harvard during installation, technical training has been great.
Review Date: 28 Jul 2021 | BTX Harvard Apparatus
The ECM 830 is a versatile electroporation system suitable for applications for gene, drug and protein delivery including: CRISPR, mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant and nuclear transfer.
ECM 830 GENERATOR
The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations.
The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.
The ECM 830 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
FEATURES
A wide range of voltages from 5 to 3,000 V
Pulse durations from 10 μsec to 999 ms
Pre-Pulse Resistance Check, Over-Current Pulse Abort, and Instrument Arc Protection Safety Features
Easy to use touchscreen user interface
Logs of every pulse delivered for QC and troubleshooting
IN VIVO APPLICATIONS
Mammalian cell transfection
In vitro, in vivo, ex vivo and in ovo tissue transfection
Nuclear transfer
Ex plant tissue transfection
Plant protoplast transfection
siRNA libraries
High throughput 25- and 96-well electroporation
ADHERENT CELL TRANSFECTION
The ECM 830 generator coupled with the Petri Pulser electrode or the adherent cell electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser electrode is ideal for 6-well plates and the adherent cell electrodes for 12-well plates.
HIGH THROUGHPUT (HT) ELECTROPORATION (45-0664 and 45-0666)
The High Throughput (HT) 96- and 25-well systems offer the advantage of multi-well electroporation technology for processing multiple samples in seconds.
This permits a large number of samples to be quickly electroporated using high throughput plate handler technology. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.
The key to the HT System is the combination of the ECM 830 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 830 generator.
MONITORING OPTION
The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each electroporation experiment.
AUTOMATIC POLARITY SWITCHING
Combine the ECM 830 with the Pulse Switcher to incorporate automatic bipolar switching into electroporation protocols. Polarity switching enhances transfection efficiency by preventing transfectant accumulation at a single electrode and enhancing delivery to the target cells or tissue.
