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ELISA-Light™ Immunoassay System

The ELISA-Light™ immunoassay system is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats) Chemiluminescent immunoassay detection allows greate…

Thermo Fisher Scientific

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Average Rating 4.5

|5Scientists have reviewed this product

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Great results

 

Average Rating 5.0

Application Area:

Analyze antibodies in blood sample quantitavely and qualitatively using microplates

Highly effective, produce reproducible results and easy to use.

Review Date: 27 Jul 2022 | Thermo Fisher Scientific

Great results.

 

Average Rating 4.0

Application Area:

Analyze enzymes/proteins

The product is good and efficient for immunoassay detection.

Review Date: 10 Jun 2020 | Thermo Fisher Scientific

Great results.

 

Average Rating 4.7

Application Area:

Inmunoglobulin 6

Equipment is very efficient, I used this equipment for researching antiasthmatics, and I recommend its use for the quantification of interleukin.

Review Date: 18 May 2020 | Thermo Fisher Scientific

Average Rating 4.7

It is effective in all the way for the Scientific and Diagnostic research use.

Review Date: 10 Nov 2015 | Thermo Fisher Scientific

Average Rating 4.3

Application Area:

biomarker study

It is easy to use. Maybe you can design ELISA plate on removable wells so that if we do not have 96 samples we don't waste whole plate.

Review Date: 31 Aug 2014 | Thermo Fisher Scientific

The ELISA-Light™ immunoassay system is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label

  • Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats)
  • Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection
  • High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary
  • A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay
  • The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used
  • Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays
  • Non-radioactive immunoassays eliminate concerns over use of radioisotopes

Extend the Limits of Detection
The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.

Multiple Substrate/Enhancer Formulations
The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate/enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate/enhancer formulations is available to facilitate assay development.

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