Eppendorf BioBLU® Single-use Vessels

Eppendorf BioBLU Single-use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design.

Single-use vessels eliminate autoclaving, improve turn-around time, simplify validation, and reduce overall costs. The BioBLU portfolio has grown to accommodate users from early research and development through manufacturing, across a wide variety of processes, including shear sensitive cell cultures, robust microbial applications, and adherent cell line development.

The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level. These rigid wall stirred-tank single-use vessels have been specifically designed for high cell density fermentation with bacteria, fungi and yeast. BioBLU f vessels can be used with the Eppendorf DASbox® and DASGIP® Parallel Bioreactor Systems.

Compared to cell culture applications, fermentation processes require much higher kLa values for proper mass transfer as well as suitable heating and cooling options. Proven stirred-tank design, powerful overhead drives featuring Rushton-type impellers and innovative baffles for cooling make the BioBLU f vessels achieve these demands.

Eppendorf BioBLU® Single-use Vessels Features:

• Market leading portfolio of single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 65 mL to 40 L working volumes.
• All BioBLU c vessel sizes available with pitched blade impellers for cell culture applications.
• BioBLU 5p vessels with packed-bed impeller, pre-loaded with Fibra-Cel® disks.
• BioBLU 0.3f and 1f vessels with Rushton-type impellers for microbial applications.
• Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement).
• Sealed magnetic drive with fully enclosed bearings maintain vessel sterility.
• Raw materials used to produce BioBLU vessels comply with USP Class VI standards and are BSE/TSE free.
• Vessels are assembled with sparger, overlay, and exhaust filters as well as penetrations for pH, DO, temperature, liquid additions, sampling and harvest.
• For use with DASbox Mini Bioreactor System, DASGIP Parallel Bioreactor Systems and New Brunswick CelliGen BLU controllers.
• Adaptor kits for legacy and 3rd party bioreactor controllers available (BioBLU 1, 5 and 14, and 50).
• Validation packages available upon request.

Applications:

• Insect, mammalian and human suspension cell lines, and stem cells Adherent cells Bacteria, yeasts and fungi.
• Adherent cells.
• Bacteria, yeasts and fungi.