Exosome Isolation

Tangential Flow Filters for EV concentration and purification

Tangential flow filtration (TFF) is a rapid and efficient method, usually used for separation and purification of biomolecules in industrial applications and can be successfully applied to isolate and separate extracellular vesicles. We provide three different typologies of filters suitable respectively for EV purification, EV concentration and EV size-based separation.

• TFF-Simple: Filter for EV concentration and dialysis: TFF-Simple is a filter cartridge in hollow fibers made of polysulfone, which allows the particle concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc..), prior to the EV purification.

Applications

• Concentration of diluted fluid as cell media or urine prior to EV isolation
• Easy removal of small molecules and ions from the EV preparation
• EV dialysis and buffer exchange
• High efficiency of EV isolation if coupled with SEC columns
• TFF-Micro: Filter for large EV separation and purification: TFF-Micro is a filter able to separate large microvesicles (MVs) by size, avoiding the separation by centrifugation at 10000g, which often causes the loss of part of small EVs. TFF-Micro retains vesicles larger than 150-200 nm, whereas small EVs and circulating molecules pass in the permeate. Retained MVs can be recovered with a syringe in PBS buffer, without additional purification steps.

Applications

• Separation, concentration and recovery of large EVs (>150 nm)
• Large EV isolation from cell media, biofluids, plant extracts
• Dialysis and desalting of large EVs
• Suitable for large EV isolation from 5 ml of fluid
• TFF-Exo: Fast EV purification: TFF-Exo is the next-generation filter which allows a rapid, reproducible and scalable purification of EVs, can be used on the lab bench for purifying small amount of samples (min 5 ml) or connected with a mechanical system for purifying larger volumes. TFF-Exo is a filter cartridge made of polyethersulfone hollow fibers with pores of 50 nm size (cut off 300 kDa). The filter allows the purification of EVs and particles > 50 nm.

Applications

• Separation, purification and concentration of EVs from conditioned media, biofluids, plant extracts
• Buffer exchange and removal of unbound dye
• Depletion of FBS from bovine EVs
• ExoSpin: Ultrafiltration concentrator: ExoSpin is a 2.5 ml non-stick ultrafiltration (UF) concentrator to enhance the recovery of extracellular vesicle (EVs) during the concentration or buffer exchange step. The ultrafiltration works in the opposite direction to the centrifugal force, providing higher particle recovery. The low protein binding membrane (polyether- sulphone) reduces the EV loss, compared to V-shape concentrators, and the reverse design of the EV-spinner ensures that the filter does not clog.
• ExoUltra: Size exclusion chromatography: Size Exclusion Chromatography (SEC) is an efficient method for isolating and purifying Extracellular Vesicles (EVs) from different fluids, not affecting the original shape and functionality of the vesicles. The range of ExoUltra SEC columns allow for EV purification from small (100 µl) to large volumes (up to 20 ml) of fluids. EV purification with the ExoUltra columns takes approximately 15 minutes allowing integration into any laboratory workflow.

Applications

• Extracellular vesicles isolation from cell media, biofluids and plant extracts
• Purification of EVs from contaminants
• Dye excess removal post EV labelling process
• ExoUltra: Immunoaffinity isolation: Understanding and addressing extracellular vesicle heterogeneity has become essential for the EV field, especially because of the different roles that EVs have in pathological processes such as cancer, infection, and neurodegenerative diseases. AMSBIO's pre-coated ELISA immunoplates and the latex or magnetic immunobeads are perfect for the capture and enrichment of total or specific EV-subpopulations.

Applications

• Multiple profiling of EV markers from both a single sample or screening a large number of samples
• EV capture and quantification from human biofluids (plasma, serum, urine, saliva)
• Immunocaptured EVs are suitable for downstream processes such as nucleic acid extraction
• ExoPure: Chemical isolation: The ExoPure is a chemical precipitation based, fast and convenient method of Extracellular Vesicle isolation from bio-fluids, cell culture supernatants and plant extracts. Samples are incubated on ice with the ExoPure solution so that EVs will precipitate following centrifugation. The obtained pellet can be re-suspended in PBS 1X or deionized water. The user-friendly protocol is time-saving and does not require expensive laboratory equipment. These isolated vesicles are suitable for isolation of EV associated nucleic acids.