Expand Long Range dNTPack
Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of…
Molecular Cloning
This expanded long PCR system ROCHE is very effective and appropriate for the long PCR product. I did amplify successfully about 8kb of PCR product with good accuracy and reliability. For constructing the targeting vector, this product was very effective and only remained with few errors. This product is best suitable for designing long PCR product.
Review Date: 10 Oct 2014
Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of thermostable DNA polymerases for use in diverse applications, including
- Genome mapping and sequencing
- Contig construction
- Characterization of cloned sequences in lambda phages or cosmids
- Cloning and analysis of eukaryotic genes
- Rapid identification and cloning of complete genes from genomic DNA using cDNA sequence as starting material
- Two-step RT-PCR with templates (up to 12 kb) reverse transcribed with our Transcriptor First Strand cDNA Synthesis Kit
Benefits
- Consistently amplify long templates that other long-fragment systems can’t amplify.
- Amplify fragments up to 25 kb.
- Obtain high yields of your desired fragment—not nonspecific products.
- Perform a wide variety of applications by using an enzyme blend that has three times greater fidelity than Taq DNA Polymerase.
- Maximize performance, economy, and ease of ordering by obtaining high-quality enzymes, buffers, and nucleotide mix simultaneously in one cost-effective dNTPack.
- Save time and resources with a convenient and flexible product.
- Use one buffer to amplify all fragment sizes.
- Fine-tune your reaction using the supplied DMSO and MgCl2 solution, if needed.
