Products & ReviewLife Sciences

EZ-96 DNA Methylation Kit

Zymo ResearchD5004, D5003

Available in both Deep-Well and Shallow-Well formats

Zymo Research

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Average Rating 4.3

|1Scientists have reviewed this product

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Zymo Kit D5004 for chip technology.

 

Average Rating 4.3

Application Area:

Illumina epic chips

We use Zymo D5004 Kit to Convert our DNA-samples for Illumina chip technology. We have used it for more than 10 years. We are very pleased with it.

Review Date: 27 Nov 2019 | Zymo Research

The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA.

Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulfonation technology eliminates otherwise cumbersome precipitations.

The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Key features include:

  • Streamlined, proven procedure for bisulfite conversion of DNA.
  • Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

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