F2H Assay Kit
F2H®-Kit Basic for effortless imaging of interactions between GFP- and RFP-tagged proteins of interest and domains thereof in live mammalian cells. The Fluorescent Two- Hybrid (F2H®) Technology is a fast and simple way to study protein-protein interactions (PPIs) by conventional fluorescence microscopy. Its direct optical readout is optimal for high-content analysis (HCA) and screening for PPI inhibitors.F2H® Principle: F…
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F2H®-Kit Basic for effortless imaging of interactions between GFP- and RFP-tagged proteins of interest and domains thereof in live mammalian cells.
The Fluorescent Two- Hybrid (F2H®) Technology is a fast and simple way to study protein-protein interactions (PPIs) by conventional fluorescence microscopy. Its direct optical readout is optimal for high-content analysis (HCA) and screening for PPI inhibitors.
F2H® Principle:
- F2H® cells (BHK origin) carry a GFP-anchoring platform in the nucleus
- GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H® cells
- GFP-bait is recruited to the platform → bright green spot
- If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot
- If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence
