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Fast-Link™ DNA Ligation Kit

Transform into E. coli without clean-up or desalting

Lucigen Corporation

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Average Rating 4.3

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Fast-Link for sure.

 

Average Rating 4.3

Application Area:

Molecular cloning

Fast-Link ligase works like a charm, easy fast and sure, what else do you need for your routine cloning work?

Review Date: 22 Mar 2021 | Lucigen Corporation

Applications

  • Blunt-end and TA cloning of PCR products
  • Ligation of next-gen sequencing adaptors to blunt-ended DNA
  • Genomic DNA cloning and subcloning
  • BAC/fosmid library construction
  • cDNA cloning
  • Linker ligation

The Fast-Link™ DNA Ligation Kit uses a high-quality ligase (Fast-Link T4 DNA Ligase), that was cloned at Epicentre and formulated to provide extremely rapid, high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. In contrast to other ligases, it is not necessary to desalt Fast-Link ligation reactions prior to the transformation of electrocompetent or chemically competent cells. The Fast-Link Kit can be used for routine and high-throughput DNA cloning.

Benefits

  • Cohesive-end ligations in 5 minutes at room temperature
  • Blunt-end ligations in 15 minutes at room temperature
  • Ligation of PCR product with A-overhangs in 1 hour at 16°C
  • High ligation efficiency
  • Desalting of ligation products is not needed prior to the transformation of electrocompetent or chemically competent cells
  • BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases

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