FastGene® qFYR Real-Time PCR System
High-precision real-time fluorescence quantitative PCR system
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The FastGene® qFYR is the highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.
The device for multiple applications
The FastGene® qFYR was developed to meet the highest laboratory standards and deliver reliable performances for various real-time qPCR applications:
- Gene expression analysis
- Absolute and relative quantification
- Endpoint qualitative analysis
- Genotyping
- Gene mutation analysis
- Pathogen detection
- GMO detection
- Protein stability screening
- miRNA analysis
- Melting curve analysis
Innovative Technology
High-sensitivity optical design
The FastGene® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.
Superior thermal cycler
The FastGene® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed-out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for the highest reaction quality and performance stability, providing reliable and consistent qPCR results.
Fast scanning mode − 4+1 channels
4 different fluorescence channels and a double FAM/SYBR channel enable fast measurements.
Due to the small distance between the scanning head and the plate, the FastGene® qFYR has an outstanding level of sensitivity and virtually no cross-talk between the individual wells.
Four different color channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 sec).
The double FAM/SYBR channel allows to perform melt curve and high resolution melt experiments in fast dual mode, which halves the measuring time in this channel.
Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.
