FiberPrep DNA Extraction kit
This DNA Extraction Kit has been designed to be used specifically with the Molecular Combing Platform. The extraction step facilitates the delivery of highly purified DNA solutions for the combing procedure. The protocol results in high molecular weight DNA (average size: 300 kb), which is an important requirement for successful combing and accurate results. The DNA Extraction Kit is designed for pelleted cells from either…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Great reagents. I can't live without it.
Isolation and stretching DNA for DNA fiber analysis
Easy protocol to use. I highly recommend for this product. Kit contains all the useful reagents you need. Save time and money to perform the experiment.
Review Date: 23 Jul 2018 | Genomic Vision
This DNA Extraction Kit has been designed to be used specifically with the Molecular Combing Platform. The extraction step facilitates the delivery of highly purified DNA solutions for the combing procedure. The protocol results in high molecular weight DNA (average size: 300 kb), which is an important requirement for successful combing and accurate results.
The DNA Extraction Kit is designed for pelleted cells from either cultured eukaryotic cell lines or purified peripheral blood leukocytes. After embedding cells in an agarose plug, proteins are digested by a proteinase and cell membranes solubilized by a surfactant, under the inhibition of DNase activity. DNA molecules are protected from mechanical stress by the agarose gel matrix. The treated DNA plugs are then available for the preparation of a high molecular weight DNA solution by agarose digestion.
