Fluorescence Microplate Assays

Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.

Many of these assays include substrates, buffers, and calibration standards as well as kinetic or endpoint protocols.

Fluorescence microplate applications

Caspase Assays

• Caspase-3/7 assays
• Caspase inhibitor-based assays

Cell Proliferation

• DNA content
• DNA synthesis

Cell Signaling and Lipids

• Cholesterol
• Phosphate and pyrophosphatase
• Phosphatase
• Phospholipase

Cell Viability

• Mammalian, yeast or bacterial cell viability
• Multiplexed viability/cytotoxicity assay

Enzyme Activity

• Phosphatases
• Phospholipases
• Proteases
• Other enzymes

β-Galactosidase Assays

• β-galactosidase activity
• β-gal substrates
• Glucosidase substrates

Ion Indicators

• Intracellular calcium
• Intracellular magnesium
• pH indicators

Metabolites and Analytes

• Metabolic assays
• Neurobiology assays
• Inflammation assays

Nucleic Acids

• dsDNA assays
• ssDNA assays
• RNA assays

Protein Quantitation

• CBQCA
• Invitrogen™ Quant-iT™ assay
• Invitrogen™ NanoOrange™ assay

Reactive Oxygen Species

• Oxidative stress
• Nitric oxide
• Other oxidative indicators

Learn more about Fluorescence Microplate Assays by Thermo Fisher Scientific here >>