G-LISA™ Small G-protein Activation Assays
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemilu…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Signaling research
Excellent kit, works every time. But it's very expensive, so I would recommend using it only when it's important to get results fast.
Review Date: 7 Jun 2012 | Cytoskeleton Inc.
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemiluminescence reagents. These assays are simple, fast (<3 hours), require only small amounts of sample (5-25 µg cell protein), yield quantitative and accurate data and are suitable for high thoughput screens.