Gel Loading Dye, Purple (6X)
Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands.
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Very Effective product.
Helps in the separation of DNA and RNA fragments according to their size and gives very clear result
I use this gel loading dye in my work experiments, and it very effectively helps in the separation of DNA and RNA fragments according to their size and gives very clear results. The best feature of this dye is that it is the combination of two dyes, red and blue, which are used for visual tracking of DNA migration while running the gel, and it is premixed with reagents so you have to simply add it with your samples before loading. Amazing dye, and highly recommended.
Review Date: 25 Jul 2022 | New England Biolabs Inc.
Gel Loading Dye, Purple (6X) is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band is the major indicator and migrates similarly to Bromophenol Blue on agarose gels. Specifically chosen, this dye does not leave a shadow under UV light. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes. This product is packaged as 4x1 ml vials.
Attention SYBR® Safe and GelRed™ dye users: Due to an increased concentration of SDS* in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
*Compared to NEB# B7021 and NEB# B7022.
