GenElute™ Bacterial Genomic DNA Kits
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria.
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Very reliable kit with consistent results.
Bacterial DNA extraction
This kit is extremely consistent and reliable for bacterial DNA extraction. While meant for genomic DNA, it also does a good job capturing plasmids of all sizes. It's easy to use and doesn't leave ethanol contamination in the final eluent like other kits we've tried. For a column-based kit, the DNA fragments are fairly long - we tried sequencing some bacterial genomes that were extracted with this kit on our ONT Minion and the average fragment length was almost 12kb for all samples.
Review Date: 11 Dec 2020 | Merck
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
The purified bacterial genomic DNA is ready for downstream applications such as:
- Restriction endonuclease digestions
- PCR
- Southern blots
- Cloning
Features and Benefits
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
