GENLISA™ Mouse Anti-SARS-CoV-2 (Covid-19) IgG Antibody to spike S1+S2 ECD protein Quantitative TITRATION ELISA
ELISA for the quantitative determination of IgG Antibodies to SARS-CoV-2 Spike S1+S2 ECD protein in mouse serum and plasma. Accurate, specific detection with reproducible results.
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Mouse Anti-SARS-COV-2 (Covid -19) IgG Antibody to spike S1+S2 ECD protein quantitative TITRATION ELISA kit is used as an analytical tool for the quantitative determination of IgG antibodies to Spike S1+S2 ECD in mouse serum.
Intended Use:
The GENLISA™ Mouse Anti-SARS-CoV-2 (2019-nCoV) IgG Antibody to Spike S1 + S2 ECD Protein Quantitative TITRATION ELISA kit is used as an analytical tool for quantitative estimation of Anti-SARS-CoV-2 (2019-nCoV) Spike S1 + S2 ECD protein in mouse serum.
Principle:
The method employs indirect sandwich ELISA technique. SARS-CoV-2 S1+S2 protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and Antibodies to Mouse Anti-SARS-CoV2 (2019-nCoV) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Anti-Mouse Anti-SARSCoV-2 (2019-nCoV) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Calibrator Range: 0 ng/ml - 720 ng/ml
- Sensitivity: 12 ng/ml
- Sample Type: Mouse serum
- Reactivity: Mouse
- Shipping Condition: 2 - 8 Deg C
- Detection Method: Colorimetric
- Usage: For Research Use Only
- Research Area: COVID-19
