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Gibson Assembly HiFi kit
The Gibson Assembly ® HiFi kit uses the original Gibson Assembly ® cloning* method, which has been optimized for the seamless joining of up to five inserts into a plasmid vector, resulting in DNA constructs with reliably low error rates.
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Codex DNA’s Gibson Assembly® HiFi kit is optimized for the scarless joining of up to five inserts into a plasmid vector, resulting in DNA constructs with low error rates. The single-tube isothermal reaction is compatible with fragments ranging from 500 bp to 32 kb and utilizes Phusion® high-fidelity DNA polymerase for 3’ end extension.