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Gibson Assembly® Cloning Kit

New England Biolabs Inc.E5510SAvailable: Worldwide

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

New England Biolabs Inc.

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Average Rating 4.5

|2Scientists have reviewed this product

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Easy and efficient assembly of multiple DNA fragments in a single reaction.

 

Average Rating 4.3

Application Area:

Assembly of DNA fragments and cloning

Gibson assembly cloning kit allows easy assembly of multiple linear DNA fragments and for use in efficient cloning.

Review Date: 18 Nov 2021 | New England Biolabs Inc.

A kit that makes complicated DNA assembly extremely easy!

 

Average Rating 4.7

Application Area:

Seamless cloning of multiple DNA fragments in a single reaction

The kit comes complete with control plasmid pieces, and along with the primer design feature on the NEB website, makes assembly of multiple pieces of DNA into a single vector extremely easy. Instructions about the length of overhangs are very clear, and it's almost fool-proof. The only thing that ever causes problems in the process is the DNA sequence itself, and the ease of designing appropriate primers for amplification.

Review Date: 17 May 2021 | New England Biolabs Inc.

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

Assembly and transformation in just under two hours

Flexible sequence design (scar-less cloning)

No PCR clean-up step required

High transformation efficiencies for inserts up to 20 kb

Easily adapted for multiple DNA manipulations, including site-directed mutagenesis

Includes competent cells

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