GS FLX Titanium Sequencing Kit XL+
Kit for determining the nucleotide sequence (in extra-long reads, up to 1kb) of an immobilized and clonally amplified DNA library prepared using the GS FLX+ System methods for the preparation of DNA libraries and amplified by the emPCR amplification process, using the appropriate GS FLX Titanium series kit(s). For use in combination with the GS FLX Titanium PicoTiterPlate Kit 70 × 75 and the GS FLX+ Instrument. Contents The GS…
De novo assembly of non model animal transcriptomes
The use of GS FLX Titanium cDNA sequencing allowed, in combination with Illumina sequencing, to produce quality assemblies and to retrieve sufficient orthologous genes between very distant non-model animal species. This product enabled the production of sequence data for comparative genomics.
Review Date: 30 Jan 2015
Kit for determining the nucleotide sequence (in extra-long reads, up to 1kb) of an immobilized and clonally amplified DNA library prepared using the GS FLX+ System methods for the preparation of DNA libraries and amplified by the emPCR amplification process, using the appropriate GS FLX Titanium series kit(s). For use in combination with the GS FLX Titanium PicoTiterPlate Kit 70 × 75 and the GS FLX+ Instrument.
Contents
The GS FLX Titanium Sequencing Kit XL+ is shipped in 7 separate containers, according to the components' shipping temperature requirements.
Package 1: Titanium Reagents XL+
Package 2: Titanium Bead and Wash Buffers
Package 3: Titanium Pre-wash Tubes XL+
Package 4: Titanium Buffer CB XL+
Package 5: Titanium Sipper Tubes XL+
Package 6: Titanium Packing Beads 70
Package 7: Titanium Supplement CB XL+
The GS FLX Titanium Sequencing Kit XL+ combined with the GS FLX Titanium PicoTiterPlate Kit 70×75 provide reagents and other consumables required for determining the nucleotide sequence (in extra-long reads; up to 1 kb) of an immobilized and clonally amplified DNA library (the sample). The sample will have been originally prepared using the GS FLX+ System methods for the preparation of DNA libraries and amplified by the emPCR amplification process, using the appropriate GS FLX Titanium series kit(s). After amplification, the DNA-carrying beads are deposited into the wells of a PicoTiterPlate (PTP) device, such that each well contains no more than a single DNA bead. The loaded PTP device is then inserted into the GS FLX+ Instrument, and sequencing reagents are sequentially flowed over the plate. Information from all the wells in the PTP device is captured simultaneously by the camera, and processed in real time by the onboard computer. The raw data consists of a set of digital images from which the sequence of the DNA library fragments is determined, and then processed as appropriate for the sample type (e.g., assembled into a consensus). The whole sequencing process can be performed by a single individual in just a few hours, in a suitably equipped laboratory.
