HbA1c

The DRG:HYBRiD-XL HbA1c is an immunoturbidimetric assay for the quantitative in vitro diagnostic measurement of Hemoglobin A1c (HbA1c) in whole blood.Only for use with the DRG:HYBRiD-XL Analyzer.HbA1c is a glycated form of haemoglobin formed by the attachment of glucose residues in the blood to the haemoglobin molecules. In the diabetic population where blood glucose levels are abnormally elevated the level of HbA1c also increases. The level of HbA1c is proportional to the level of glucose in the blood and has been widely accepted as an indicator of the mean blood glucose concentration in the preceding 6-8 weeks. It is therefore a long-term indicator of diabetic control. For routine use HbA1c levels should be monitored every 3-4 months. However in gestational diabetes and after a change in therapy it may be useful to measure HbA1c more frequently at 2-4 week intervals.The DRG:HYBRiD-XL HbA1c is an immunoturbidimetric assay. The sample (haemolysis sample) is added to the unsensitised latex particles, and the surface of the latex adsorbs total haemoglobin in the sample. Anti-human HbA1c mouse monoclonal antibodies interact with the latex-bound HbA1c. Reaction with anti-mouse IgG goat antibody leads to an amplification of this reaction and the forming of agglutination complexes. The amount of agglutination caused depends on the amount of HbA1c that adsorbs the surface of the latex, this agglutination is measured as a turbidity. The concentration of HbA1c (mmol/mol) in the sample is determined by referring to the calibration curve.