Products & ReviewLife Sciences
HiDi Taq 2x PCR Master Mix
Highly discriminatative master mix for mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use aptamer-based fast-start formulated master mix specially optimized for assays in which high discrimination is required. With this unique mix less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.
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- Unique. Engineered thermostable Taq DNA polymerase with reverse transcriptase activity reading both RNA and DNA Extremely thermostable with half-life at 95°C of >40 min.
- Fast Time-to-Result. No isothermal reverse transcription step required. Fast “zero-step” RT-PCRs directly from RNA
- No extraction needed. Immediate hot PCR cycling conditions can break cell and virus membranes
- No false amplification. Aptamer based hot-start prevents false amplification and provides a fast-start function
- Easy handling. Fully equipped ready-to-use reaction mix. Blue color as pipetting aid for better visibility
- Cited by Top scientific Journals e.g Nature Cell Death & Disease, Stem Cell Report, plos pathogens