Products & ReviewLife Sciences
High Pure miRNA Isolation Kit
The High Pure miRNA Isolation Kit purifies and enriches small RNAs, such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed, paraffin-embedded sections) or plant material
Kit for 50 isolations
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The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:
- Northern blotting
- cDNA synthesis
- Primer extension
- miRNA array hybridization
- Relative quantification of miRNA with RT-PCR using, for example, the LightCycler® 480 System
Features and Benefits
- Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription; ideal for qualitative and quantitative reverse transcription.
- Obtain excellent performance and linearity in RT-PCR.
- Generate stable, highly pure miRNAs using a simple, efficient protocol.
- Purify miRNA without using hazardous organic solvents. Avoid phenol/chloroform steps required by other suppliers′ miRNA purification kits.
- Choose one flexible kit for all your miRNA purifications. Use the same kit to purify small RNAs from a variety of sample types.
Quality
More than 60% recovery is obtained when 1 μg miRNA is added to106 K-562 cells and isolated with the one-column method, and more than 50% is recovered with the two-column method.
