High Pure PCR Product Purification Kit
The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers, mineral oil, salts, unincorporated nucleotides, and the thermostable DNA polymerase may inhibit subsequent enzymatic reactions (e.g., labeling, sequencing or cloning of the PCR products). In addition, nucleic acids from other modification reactions (e.g., restriction-endonuclease digests, alkaline-phosphatase t…
The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers, mineral oil, salts, unincorporated nucleotides, and the thermostable DNA polymerase may inhibit subsequent enzymatic reactions (e.g., labeling, sequencing or cloning of the PCR products).
In addition, nucleic acids from other modification reactions (e.g., restriction-endonuclease digests, alkaline-phosphatase treatment, or kinase reactions) can be purified using this kit. It can also be applied to concentrate dilute nucleic acid solutions.
Benefits
- Obtain high yields.
Starting material is purified with high recovery by avoiding the substantial DNA loss caused by precipitation-based methods. - Save time.
Multiple PCR products can be purified in parallel in 10 minutes. - Take advantage of a convenient kit.
Easy-to-use spin columns containing glass fiber fleece eliminate contaminants and short DNA fragments (<100 bp). - Elute in low-salt buffer.
The purified DNA can be used directly in cloning, sequencing, and other downstream applications.
