High Pure RNA Tissue Kit
The High Pure RNA Tissue Kit is designed to purify total, intact RNA from tissue samples (e.g., mouse liver, spleen, lung, heart) for use in research. The isolated RNA may be used directly for RT-PCR, northern blotting, and RNase protection assays. PrincipleAs a prerequisite for the reverse transcription polymerase chain reaction (RT-PCR), intact RNA must be isolated from tissue. Tissue samples are homogenized in the presence…
The High Pure RNA Tissue Kit is designed to purify total, intact RNA from tissue samples (e.g., mouse liver, spleen, lung, heart) for use in research. The isolated RNA may be used directly for RT-PCR, northern blotting, and RNase protection assays.
Principle
As a prerequisite for the reverse transcription polymerase chain reaction (RT-PCR), intact RNA must be isolated from tissue. Tissue samples are homogenized in the presence of a strong denaturing buffer to inactivate RNases and ensure the isolation of intact RNA. After the addition of ethanol, RNA binds selectively to a glass fiber fleece in the presence of chaotropic salts. Residual contaminating DNA is digested by DNase I and applied directly on the glass fiber fleece. The binding process is specific for nucleic acids, which are purified from salts, proteins, and cellular components by repeated washing and centrifugation steps. The bound RNA is eluted in water.
