High Pure Viral Nucleic Acid Kit

The High Pure Nucleic Acid Kit is intended for general laboratory use and is designed to purify viral nucleic acids from serum, plasma, or whole blood, using sample volumes of up to 200 µl. When using whole blood, total nucleic acids are isolated, including viral nucleic acids. The purified viral nucleic acid is used for PCR and RT-PCR analysis directly after elution in nuclease-free water.

Principle

As a prerequisite for PCR or RT-PCR, viral nucleic acids must be isolated from serum, plasma, or whole blood. Virus lysis is accomplished by incubation of the sample in a special Lysis/Binding Buffer in the presence of Proteinase K. After applying the lysis mixture to a High Pure Filter Tube, nucleic acids bind to the surface of glass fibers in the presence of chaotropic salts. The binding process is specific for nucleic acids, which are purified from salts, proteins, and other impurities by a washing and centrifugation step. The bound nucleic acids are eluted in nuclease-free water, and collected after centrifugation.