High Select™ Top14 Abundant Protein Depletion Resin
Thermo Scientific High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Essential part of our sample preparation pipeline for mass spectrometry.
Mass spectrometry - sample preparation
When performing discovery proteomics you need to immunodeplete the most abundant proteins to be able to detect and quantify less abundant proteins. Top 14 resin allows us to complete this process. As it is sold as a resin, it is suitable to be used in the 96-plate format that increases efficiency. For us in the laboratory Top 14 resin is essential and it is highly recommended. It is easy to use and the price is worth paying. We obtain reproducible results which are important for our downstream workflows.
Review Date: 28 Apr 2021 | Thermo Fisher Scientific
Features of Top14 Abundant Protein Depletion Resin include:
- Flexible—provided in bulk format for depletion of user-defined amount of human plasma sample
- Optimized—remove >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14)
- Fast—process samples in ~10 minutes
- Economical—cost-effective bulk format
High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids. The resin is provided in an economical bulk format that may be used to fill columns or plates, depending on sample volumes.
Analysis of human fluids is often complicated by the presence of high concentrations of albumin and IgG that can make up more than 70% of total serum protein. Removal of these proteins is often essential for the study of low-abundant proteins. High Select Top14 Abundant Protein Depletion Resin can deplete greater than 95% of the 14 most abundant proteins in serum. Columns or plates are filled with the resin based on the needs of the user, and samples processed in approximately 10 minutes. The depletion of highly abundant proteins enables the detection of low abundant proteins in samples and subsequent identification by mass spectrometry or 1D or 2D gel electrophoresis.
