HiScript II Reverse Transcriptase
HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved.
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HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved.
The half-life of HiScript II Reverse Transcriptase at 50℃ is >240 min. At 55℃, it can also be stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structure. In addition, it has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).
Product advantages
- The half-life is more than 240 min at 50℃ and 60 min at 55℃
- The length of 10 KB cDNA can be synthesized in 5 min, which is suitable for rapid reverse transcription
- Higher tolerance of impurities, higher detection sensitivity, molecular diagnosis preferred reverse transcriptase
Applications
It is applicable for reverse transcription of animal, plant and microbial RNA.
Source
It is cloned from M-MLV with improved reverse transcriptase gene and purified from E.coli.
Unit definition
One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10 min at 37℃ with Poly (rA)-Oligo (dT) as the template/primer.
