HiScript III U+ One Step qRT-PCR Probe Kit
HiScript III U+ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.
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HiScript III U+ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature.
It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III U+ One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III U+ One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step U+ Mix contains an optimized buffer and dNTP/dUTP Mix, and is suitable for high-specificity detection systems based on flfluorescence labelled probes (e.g. TaqMan).
Product advantages
- Higher detection sensitivity
- Suitable for multiple qPCR
- Compatible fast program
- Superior storage stability
- High and low template concentrations amplify evenly
- Introduce dUTP/UDG anti-contamination system
Applications
This product is suitable for detection of various RNA nucleic acids of animals, plants and microorganisms (viruses, etc.).
Notes
One Step U+ Enzyme Mix contains high concentration of glycerol. Please centrifuge briefly and mix gently before use. To avoid contamination, please use RNase-free tips and EP tubes.
